Difference between revisions of "Part:BBa K190028:Experience"

Revision as of 19:55, 20 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K190028

This part has been used to import As(III) into the E.coli cells. It has been used in combination with metal accumulation proteins to make a accumulation device. The two devices are BBa_K190038, a combination of GlpF with the accumulatin protein [http://2009.igem.org/Team:Groningen/Project/Accumulation#Metals fMT] and BBa_K190037, a combination of GlpF with the fusion protein of MBP with [http://2009.igem.org/Team:Groningen/Project/Accumulation#Metals ArsR] (an accumulation protein).

User Reviews

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iGEM_Groningen2009

We did not have a lot of trouble with doing a pcr of GlpF to get it out of the genome or to get the EcoR1 and Pst1 site out. We used it in testing the import of arsenic by doing a accumulation assay and analyse it with ICP-MS. Protocols can be found [http://2009.igem.org/Team:Groningen/Protocols here]

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iGEM Groningen 2009 The capacity of GlpF to enhance the arsenite accumulation in E. coli was tested using an arsenite uptake [http://2009.igem.org/Team:Groningen/Protocols assay]. But unfortunately the arsenic concentration determined by ICP-MS was not reproducible and did not show enhanced arsenite accumulation in E. coli carrying GlpF (induced by lactose).

UNIQc0f6e200ec6e2856-partinfo-00000004-QINU UNIQc0f6e200ec6e2856-partinfo-00000005-QINU

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 We did not have a lot of trouble with doing a pcr of GlpF to get it out of the genome or to get the EcoR1 and Pst1 site out. We used it in testing the import of arsenic by doing a accumulation assay and analyse it with ICP-MS. Protocols can be found [http://2009.igem.org/Team:Groningen/Protocols here]
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+<partinfo>BBa_K190028 AddReview 2</partinfo>
+<I>iGEM Groningen 2009</I>
+The capacity of GlpF to enhance the arsenite accumulation in ''E. coli'' was tested using an arsenite uptake [http://2009.igem.org/Team:Groningen/Protocols assay]. But unfortunately the arsenic concentration determined by ICP-MS was not reproducible and did not show enhanced arsenite accumulation in ''E. coli'' carrying GlpF (induced by lactose).
+|}