Difference between revisions of "Part:BBa K4825006:Design"
Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
− | Saccharomyces cerevisiae | + | <i>Saccharomyces cerevisiae</i> |
===References=== | ===References=== | ||
Aza P, Molpeceres G, de Salas F, Camarero S. Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast. Cell Mol Life Sci. 2021 Apr;78(7):3691-3707. doi: 10.1007/s00018-021-03793-y. Epub 2021 Mar 9. PMID: 33687500; PMCID: PMC8038962. | Aza P, Molpeceres G, de Salas F, Camarero S. Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast. Cell Mol Life Sci. 2021 Apr;78(7):3691-3707. doi: 10.1007/s00018-021-03793-y. Epub 2021 Mar 9. PMID: 33687500; PMCID: PMC8038962. |
Latest revision as of 11:51, 12 October 2023
OPT
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 268
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 268
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 268
Illegal XhoI site found at 244 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 268
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 268
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A9D and A20T mutations are made from the signal peptide ScMα to improve the production of heterologous proteins in S.cerevisiae as a universal signal peptide.
Source
Saccharomyces cerevisiae
References
Aza P, Molpeceres G, de Salas F, Camarero S. Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast. Cell Mol Life Sci. 2021 Apr;78(7):3691-3707. doi: 10.1007/s00018-021-03793-y. Epub 2021 Mar 9. PMID: 33687500; PMCID: PMC8038962.