Difference between revisions of "Part:BBa K4808008"

Revision as of 11:25, 12 October 2023

thrABC

In Escherichia coli, thrA, thrB and thrC are arranged adjacently on the chromosome to form the thrABC operon. The thrABC operon controls several key enzymes, from aspartate to L-threonine synthesis. Research on thrABC expression and regulation is important for L-threonine synthesis and the downstream products, L-isoleucine and L-glycine. Overexpression of the thrABC operon in a bacterial strain increased L-threonine production significantly.

In our project, we increased the copy number of thrABC in E. coli to increase the production of a-KB.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 3506
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 3506
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 3506
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 3506
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 3506
Illegal NgoMIV site found at 403
Illegal NgoMIV site found at 3357
Illegal AgeI site found at 912
Illegal AgeI site found at 1839
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 3614

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4808008 short</partinfo>
-ABC
+In Escherichia coli, thrA, thrB and thrC are arranged adjacently on the chromosome to form the thrABC operon. The thrABC operon controls several key enzymes, from aspartate to L-threonine synthesis. Research on thrABC expression and regulation is important for L-threonine synthesis and the downstream products, L-isoleucine and L-glycine. Overexpression of the thrABC operon in a bacterial strain increased L-threonine production significantly.
+In our project, we increased the copy number of thrABC in E. coli to increase the production of a-KB.
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