Difference between revisions of "Part:BBa K4591013"

Latest revision as of 11:23, 12 October 2023

T7-DepoPETase-T500

Usage and Biology

This circuit contains the coding sequences of P₄₃, DepoPETase, and MHETase. The expression of enzyme genes related to this route is controlled by the frequently expressed promoter P₄₃, which degrades PET into TPA and achieves the preliminary degradation of PET.

Basic components of the circuit

P₄₃：P₄₃is a constitutive promoter that constitutively expresses the P₄₃ protein in B.subtilis.

DepoPETase：DepoPETase is the best mutant obtained by researchers using a novel high-throughput screening method based on fluorescence detection for directed evolution of IsPETase.

MHETase：MHETase is discovered from Ideonella sakaiensis 201-F6, it is a enzyme which can hydrolyze MHET, which is the main product of PETase when hydrolyzing PET.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 469
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 239
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 25

@@ Line 6: / Line 6: @@
 ===Basic components of the circuit===
-<p><i>P</i><sub>43</sub>：<i>P</i><sub>43</sub>is a constitutive promoter that constitutively expresses the P<sub>43</sub> protein in <i>B.subtilis.</i> </p><p>
+<p><i>P</i><sub>43</sub>：<i>P</i><sub>43</sub>is a constitutive promoter that constitutively expresses the <i>P</i><sub>43</sub> protein in <i>B.subtilis.</i> </p><p>
 DepoPETase：DepoPETase is the best mutant obtained by researchers using a novel high-throughput screening method based on fluorescence detection for directed evolution of IsPETase.</p><p>
 MHETase：MHETase is discovered from Ideonella sakaiensis 201-F6, it is a enzyme which can hydrolyze MHET, which is the main product of PETase when hydrolyzing PET.</p>