Difference between revisions of "Part:BBa K4790108"

 
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<partinfo>BBa_K4790108 short</partinfo>
 
<partinfo>BBa_K4790108 short</partinfo>
  
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Node 98 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node 98, and find variants with excellent properties.
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Node 98 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node98, and find variants with excellent properties.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:13, 12 October 2023


ASR2(node 98)

Node 98 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node98, and find variants with excellent properties.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 472
    Illegal PstI site found at 928
    Illegal PstI site found at 1129
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 472
    Illegal PstI site found at 928
    Illegal PstI site found at 1129
    Illegal NotI site found at 758
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 336
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 472
    Illegal PstI site found at 928
    Illegal PstI site found at 1129
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 472
    Illegal PstI site found at 928
    Illegal PstI site found at 1129
    Illegal NgoMIV site found at 294
    Illegal NgoMIV site found at 1035
    Illegal NgoMIV site found at 1416
  • 1000
    COMPATIBLE WITH RFC[1000]