Difference between revisions of "Part:BBa K4790070"

Revision as of 11:11, 12 October 2023

ASR5(node 111)

Node 111 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node111, and find variants with excellent properties.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 286
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 205
Illegal PstI site found at 286
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 286
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 286
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4790070 short</partinfo>
-Node 111 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node 111, and find variants with excellent properties.
+Node 111 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node111, and find variants with excellent properties.
 <!-- Add more about the biology of this part here