Difference between revisions of "Part:BBa K4790069"

Revision as of 11:10, 12 October 2023

ASR4(node 109)

Node 109 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node109, and find variants with excellent properties.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 289
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 289
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 289
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 289
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4790069 short</partinfo>
-Node 109 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node 109, and find variants with excellent properties.
+Node 109 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node109, and find variants with excellent properties.
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