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| | ==='''MEASUREMENT1'''=== | | ==='''MEASUREMENT1'''=== |
| − | *[[K176126]] is a device which can response to different concentrations of AHL. Here, we use K176126 as a detector to examing the AHL concentrations produced by '''constitutive promoters + luxI'''. | + | *K176126([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K176126]]) is a device which can response to different concentrations of AHL. Here, we use K176126 as a detector to examing the AHL concentrations produced by '''constitutive promoters + luxI'''. |
| | | | |
| | '''constitutive promoters + luxI-LVA''': | | '''constitutive promoters + luxI-LVA''': |
Revision as of 19:07, 20 October 2009
MEASUREMENT1
- K176126([[1]]) is a device which can response to different concentrations of AHL. Here, we use K176126 as a detector to examing the AHL concentrations produced by constitutive promoters + luxI.
constitutive promoters + luxI-LVA:
K176020( J23101->luxI-LVA);
K176021( J23107->luxI-LVA);
K176022( J23115->luxI-LVA);
K176023( J23109->luxI-LVA);
K176024( J23103->luxI-LVA);
measuring device:
K176217( K176020 + K176126);
K176218( K176021 + K176126);
K176219( K176022 + K176126);
K176220( K176023 + K176126);
K176221( K176024 + K176126);
data
|
K176217
| T(min)
|
OD600
|
FLU(arbitrary)
|
FLU/OD600(arbitrary)
|
ST.FLU/OD600(ustc_st1)
|
ST.FLU(ustc_st1)
|
| 0 |
0.0875 |
100.2605 |
1147.557098 |
4587.109158 |
401.3720513
|
| 60 |
0.120666667 |
148.52 |
1229.380903 |
4914.181969 |
592.9779576
|
| 120 |
0.145 |
198.92 |
1370.07619 |
5476.580687 |
794.1041996
|
| 180 |
0.207666667 |
292.4516667 |
1404.89787 |
5615.772756 |
1166.208809
|
|
K176218
| T(min)
|
OD600
|
FLU(arbitrary)
|
FLU/OD600(arbitrary)
|
ST.FLU/OD600(ustc_st1)
|
ST.FLU(ustc_st1)
|
| 0 |
0.1035 |
75.11366667 |
721.4097924 |
2883.678268 |
298.4607008
|
| 60 |
0.150333333 |
123.0506667 |
808.6659075 |
3232.465553 |
485.9473215
|
| 120 |
0.1995 |
195.035 |
966.403576 |
3862.987472 |
770.6660008
|
| 180 |
0.308333333 |
351.1483333 |
1131.802978 |
4524.135501 |
1394.941779
|
|
K176219
| T(min)
|
OD600
|
FLU(arbitrary)
|
FLU/OD600(arbitrary)
|
ST.FLU/OD600(ustc_st1)
|
ST.FLU(ustc_st1)
|
| 0 |
0.2515 |
32.2705 |
125.5594805 |
501.8966322 |
126.227003
|
| 60 |
0.372833333 |
43.24566667 |
112.4353648 |
449.4358429 |
167.5646634
|
| 120 |
0.474833333 |
68.484 |
139.8281768 |
558.9326329 |
265.3998452
|
| 180 |
0.594833333 |
116.7865 |
192.791143 |
770.6405364 |
458.4026791
|
|
K176220
| T(min)
|
OD600
|
FLU(arbitrary)
|
FLU/OD600(arbitrary)
|
ST.FLU/OD600(ustc_st1)
|
ST.FLU(ustc_st1)
|
| 0 |
0.2485 |
7.723 |
32.1438897 |
128.4881869 |
31.92931443
|
| 60 |
0.4105 |
12.202 |
29.72814969 |
118.8317932 |
48.78045109
|
| 120 |
0.491666667 |
17.4355 |
36.20398381 |
144.7175273 |
71.15278427
|
| 180 |
0.611 |
31.26466667 |
51.84736322 |
207.2485239 |
126.6288481
|
|
K176221
| T(min)
|
OD600
|
FLU(arbitrary)
|
FLU/OD600(arbitrary)
|
ST.FLU/OD600(ustc_st1)
|
ST.FLU(ustc_st1)
|
| 0 |
0.219666667 |
11.49566667 |
52.33304675 |
209.1899378 |
45.95205634
|
| 60 |
0.3495 |
16.41366667 |
46.97848701 |
187.7862534 |
65.63129556
|
| 120 |
0.454833333 |
24.69433333 |
54.29412657 |
217.0289266 |
98.71199013
|
| 180 |
0.5765 |
43.4475 |
75.41615552 |
301.4596295 |
173.7914764
|
For the details of the definition of ST.FLU and the unit ustc_st1, please click here.
plot
 Figure 1- the relationship chart between FLU and OD600 of diffrent constitutive promoters + luxI-LVA  Figure 2- The time response of K176126 to different constitutive promoters + luxI-LVA. We can see from the figure that the more strong the constitutive promoter is , the more strong response it shows. |
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