Difference between revisions of "Part:BBa K4837000:Design"
(→Design Notes) |
|||
| Line 18: | Line 18: | ||
pSBIK3 vector was adopted in this plasmid.The promoter used in these two basic parts was arabinose promoter. Purification tag and fluorescent tag were also included for better detection of the optimized enzyme synthesized. | pSBIK3 vector was adopted in this plasmid.The promoter used in these two basic parts was arabinose promoter. Purification tag and fluorescent tag were also included for better detection of the optimized enzyme synthesized. | ||
| − | Other biobricks such as BBa_K4837001 are required to perform its function. | + | Other biobricks such as BBa_K4837001 are required to perform its function. The details of these two basic parts are listed below: |
| + | |||
| + | <html> | ||
| + | <a href='https://static.igem.wiki/teams/4837/wiki/part-table.jpg' target="_blank"><img src="https://static.igem.wiki/teams/4837/wiki/part-table.jpg" width="100%"/> | ||
| + | </a> | ||
| + | </html> | ||
| + | |||
===Source=== | ===Source=== | ||
Latest revision as of 10:50, 12 October 2023
Lignin peroxidase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 126
Illegal NheI site found at 1694 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 66
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 260
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2352
Design Notes
The biobricks contained in our plasmid are illustrated in the above graph. Two basic parts we designed, including BBa_K4837000 and BBa_K4837001 are used to synthesize lignin peroxidase (LiP) and manganese peroxidase (MnP) respectively.
pSBIK3 vector was adopted in this plasmid.The promoter used in these two basic parts was arabinose promoter. Purification tag and fluorescent tag were also included for better detection of the optimized enzyme synthesized.
Other biobricks such as BBa_K4837001 are required to perform its function. The details of these two basic parts are listed below:
Source
It is designed based on part of Phanerochaete chrysosporium.