Difference between revisions of "Part:BBa K243017"

Revision as of 18:51, 20 October 2009

Strep-DigA-Split Linker-Fok_a

This part is the counter part to [BBa_K243010],it inhabits the active cutting site of our universal endonuclease. We prefer this part because it has an other combiantion of basic parts than BBa_K243010, that allows us another way of purification and linkage to the labeled oligos, which were hybridized with the target DNA.[explication] The purification is made with a Streptavidin column.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 278
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1132

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K243017 short</partinfo>
-These part units some of our parts. The combination of a Histidin-Tag, a Lipocalin-Tag and the linked protein domain foka, make it possible to purifier and detect the function of the protein in one step. The fluorescineA tag allows the measurement by quenching and the coupling to a anticalin linked oligo.
+This part is the counter part to [BBa_K243010],it inhabits the active cutting site of our universal endonuclease.
+We prefer this part because it has an other combiantion of basic parts than BBa_K243010, that allows us another way of purification and linkage to the labeled oligos, which were hybridized with the target DNA.[explication]
+The purification is made with a Streptavidin column.
 <!-- Add more about the biology of this part here