Difference between revisions of "Part:BBa K4790089"

 
 
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<partinfo>BBa_K4790089 short</partinfo>
 
<partinfo>BBa_K4790089 short</partinfo>
  
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The DNA fragment has been site-directed mutated through PCR. The 177th amino acid in expression product of node 59 was mutated from proline to glutamic.
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ASR1(node 59)-P177E is constructed on pET-22b(+) vector by Gibson Assembly method. DNA fragment of ASR1(node 59)-P177E was subcloned into RBS site between T7 promoter and T7 terminator. The antibiotic marker of pET-22b is the ampicillin resistance gene.
  
 
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Latest revision as of 10:46, 12 October 2023


ASR1(node 59)-P177E/pET-22b(+)

ASR1(node 59)-P177E is constructed on pET-22b(+) vector by Gibson Assembly method. DNA fragment of ASR1(node 59)-P177E was subcloned into RBS site between T7 promoter and T7 terminator. The antibiotic marker of pET-22b is the ampicillin resistance gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 960
    Illegal PstI site found at 261
    Illegal PstI site found at 393
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 960
    Illegal NheI site found at 882
    Illegal NheI site found at 1113
    Illegal PstI site found at 261
    Illegal PstI site found at 393
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 960
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 960
    Illegal PstI site found at 261
    Illegal PstI site found at 393
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 960
    Illegal PstI site found at 261
    Illegal PstI site found at 393
    Illegal NgoMIV site found at 143
  • 1000
    COMPATIBLE WITH RFC[1000]