Difference between revisions of "Part:BBa K4790085"

 
 
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<partinfo>BBa_K4790085 short</partinfo>
 
<partinfo>BBa_K4790085 short</partinfo>
  
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ASR4 is constructed based on pET-22b. We amplified pET-22b by PCR and use Gibson Assembly method. DNA fragment node 111 was subcloned into pET-22b vector between T7 promoter and T7 terminator. The antibiotic marker of pET-22b is ampicillin resistance gene.
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ASR5(node 111) is constructed on pET-22b(+) vector by Gibson Assembly method. DNA fragment of node 111 was subcloned into RBS site between T7 promoter and T7 terminator. The antibiotic marker of pET-22b is the ampicillin resistance gene.
  
 
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Latest revision as of 10:41, 12 October 2023


ASR5(node 111)/pET-22b(+)

ASR5(node 111) is constructed on pET-22b(+) vector by Gibson Assembly method. DNA fragment of node 111 was subcloned into RBS site between T7 promoter and T7 terminator. The antibiotic marker of pET-22b is the ampicillin resistance gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 447
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 366
    Illegal NheI site found at 945
    Illegal PstI site found at 447
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 447
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 447
    Illegal NgoMIV site found at 143
  • 1000
    COMPATIBLE WITH RFC[1000]