Difference between revisions of "Part:BBa K265011"

Revision as of 18:34, 20 October 2009

OmpA with proteolytic cleavage tag

This part is a fusion of a modified OmpA with a signal sequence to allow cleavage when a protein is fused. OmpA was modified by replacing the normal restriction sites with sites for RFC12 fusion format. An OmpA/protein fusion normally allows display of proteins on the cell surface by way of a linker. The addition of this signal sequence allows secretion by cleavage from the OmpA linker after display.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K265011 short</partinfo>
-This part is a fusion of a modified OmpA with a signal sequence to allow cleavage when a protein is fused. OmpA was modified by replacing the normal restriction sites with Tom Knight's fusion format.
+This part is a fusion of a modified OmpA with a signal sequence to allow cleavage when a protein is fused. OmpA was modified by replacing the normal restriction sites with sites for RFC12 fusion format.
-An OmpA/protein fusion normally allows display of proteins on the cell surface. The addition of this signal sequence allows secretion by allowing cleavage after display.
+An OmpA/protein fusion normally allows display of proteins on the cell surface by way of a linker. The addition of this signal sequence allows secretion by cleavage from the OmpA linker after display.
 <!-- Add more about the biology of this part here