Difference between revisions of "Part:BBa K4790109"

 
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<partinfo>BBa_K4790109 short</partinfo>
 
<partinfo>BBa_K4790109 short</partinfo>
  
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ASR2 is constructed based on pET-22b. We amplified pET-22b by PCR and use Gibson Assembly method. DNA fragment node 98 was subcloned into pET-22b vector between T7 promoter and T7 terminator. The antibiotic marker of pET-22b is ampicillin resistance gene.
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ASR2 （node 98） is constructed on pET-22b（+）vector by Gibson Assembly method. DNA frament of ASR2(node 98） was subcloned into RBS site between T7 promoter and T7 terminator. The antibiotic marker of pET-22b is ampicilin resistance gene.
  
 
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Revision as of 10:28, 12 October 2023


ASR2(node 98)/pET-22b(+)

ASR2 （node 98） is constructed on pET-22b（+）vector by Gibson Assembly method. DNA frament of ASR2(node 98） was subcloned into RBS site between T7 promoter and T7 terminator. The antibiotic marker of pET-22b is ampicilin resistance gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 635
    Illegal PstI site found at 1091
    Illegal PstI site found at 1292
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1967
    Illegal PstI site found at 635
    Illegal PstI site found at 1091
    Illegal PstI site found at 1292
    Illegal NotI site found at 921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 499
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 635
    Illegal PstI site found at 1091
    Illegal PstI site found at 1292
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 635
    Illegal PstI site found at 1091
    Illegal PstI site found at 1292
    Illegal NgoMIV site found at 143
    Illegal NgoMIV site found at 457
    Illegal NgoMIV site found at 1198
    Illegal NgoMIV site found at 1579
  • 1000
    COMPATIBLE WITH RFC[1000]