Difference between revisions of "Part:BBa K265009"

 
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<partinfo>BBa_K265009 short</partinfo>
 
<partinfo>BBa_K265009 short</partinfo>
  
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It is believed that when coupled with the correct signal sequence, INPNC will secrete better.
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This composite part is comprised of the ice-nucleation protein, INPNC, and a proteolytic cleavage sequence for secreting target proteins into the extracellular region.  This combination will give optimal yield since the cleavage sequence aids in transporting the target protein to the inner membrane where the INPNC can further transport the protein to the cell surface.  See parts BBa_K265002 and BBa_K265008 for more information.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 18:28, 20 October 2009

Ice Nucleation Protein NC + Proteolytic Cleavage Sequence

This composite part is comprised of the ice-nucleation protein, INPNC, and a proteolytic cleavage sequence for secreting target proteins into the extracellular region. This combination will give optimal yield since the cleavage sequence aids in transporting the target protein to the inner membrane where the INPNC can further transport the protein to the cell surface. See parts BBa_K265002 and BBa_K265008 for more information.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 405
  • 1000
    COMPATIBLE WITH RFC[1000]