Difference between revisions of "Part:BBa K4601004"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | NpHR is a halorhodopsin identified in the archaea ''Natronomonas pharaonis'' (Uniprot P15647). Its a mutant P240T, F250Y is able to interact with NpHtrII, while the wild type protein (BBa_K4601003) is not [1]. | + | NpHR is a halorhodopsin identified in the archaea ''Natronomonas pharaonis'' (Uniprot P15647). Its a mutant P240T, F250Y is able to interact with NpHtrII, while the wild type protein ([[Part:BBa_K4601003|BBa_K4601003]]) is not [1]. |
Halorhodopsins are chloride inward pumps activated by light [2]. | Halorhodopsins are chloride inward pumps activated by light [2]. | ||
Revision as of 10:09, 12 October 2023
NpHR P240T F250Y
This part is the NpHR P240T F250Y mutant halorhodopsin.
Usage and Biology
NpHR is a halorhodopsin identified in the archaea Natronomonas pharaonis (Uniprot P15647). Its a mutant P240T, F250Y is able to interact with NpHtrII, while the wild type protein (BBa_K4601003) is not [1].
Halorhodopsins are chloride inward pumps activated by light [2].
For the characterisation of this part an expression cassette was assembled and the results are presented on its page in Parts Registry: BBa_K4601204.
References
[1] Sudo Y, Yamabi M, Kato S, Hasegawa C, Iwamoto M, Shimono K, Kamo N. Importance of specific hydrogen bonds of archaeal rhodopsins for the binding to the transducer protein. Journal of Molecular Biology (2006) 357: 1274–1282.
[21] Engelhard C, Chizhov I, Siebert F, Engelhard M. Microbial halorhodopsins: light-driven chloride pumps. Chemical Reviews (2018) 118: 10629–10645.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]