Difference between revisions of "Part:BBa K4790029"

 
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<partinfo>BBa_K4790029 short</partinfo>
 
<partinfo>BBa_K4790029 short</partinfo>
  
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The DNA fragment has been site-directed mutated through PCR. The 223th amino acid in expression product of node 59 was mutated from isoleucine to glutamate.
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Mutate I223 of ASR1 (Node 59) to E223 by site-directed mutagenesis.
  
 
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Revision as of 09:58, 12 October 2023


ASR1(node 59)-I223E

Mutate I223 of ASR1 (Node 59) to E223 by site-directed mutagenesis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 799
    Illegal NheI site found at 721
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 799
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 1000
    COMPATIBLE WITH RFC[1000]