Difference between revisions of "Part:BBa K4814012"

Revision as of 09:55, 12 October 2023

Strong RBS

To improve the RecA (K3) - B0034 - EGFP bioreporter we designed to test RecA(K3) (BBa_K3020001) promoter, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates. Sequence: TTTAAGAGGGGGCTATACAT

Click here for the experiment data: RecA(K3)-Strong-eGFP BBa_K4814013

References:

Zhang, M., Holowko, M. B., Zumpe, H. H., & Ong, C. S. (2022). Machine Learning Guided Batched Design of a Bacterial Ribosome Binding Site. ACS Synthetic Biology, 11(7), 2314-2326. https://doi.org/10.1021/acssynbio.2c00015

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4814012 short</partinfo>
-To attempt to improve the RecA (K3) - B0034 - EGFP bioreporter we designed to test RecA(K3) (BBa_K3020001) promoter, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates.
+To improve the RecA (K3) - B0034 - EGFP bioreporter we designed to test RecA(K3) (BBa_K3020001) promoter, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates.
 Sequence: TTTAAGAGGGGGCTATACAT