Difference between revisions of "Part:BBa K4814011"
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We have used the codon optimized version of mCherry by Shaner, N. et al (2004), derived from the Discosoma (DsRed). This protein is used to construct a FRET pair to detect DNA damage in mammalian cells. Please refer to BBa_K4814006. | We have used the codon optimized version of mCherry by Shaner, N. et al (2004), derived from the Discosoma (DsRed). This protein is used to construct a FRET pair to detect DNA damage in mammalian cells. Please refer to BBa_K4814006. | ||
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| + | Click here for the experiment data: <html><a href="https://parts.igem.org/Part:BBa_K4814007">RPA1-mCherry BBa_K4814007</a></html> | ||
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| + | References | ||
Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N., Palmer, A. E., & Tsien, R. Y. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature biotechnology, 22(12), 1567–1572. https://doi.org/10.1038/nbt1037 | Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N., Palmer, A. E., & Tsien, R. Y. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature biotechnology, 22(12), 1567–1572. https://doi.org/10.1038/nbt1037 | ||
Latest revision as of 09:52, 12 October 2023
Mammalian Codon Optimized mCherry
We have used the codon optimized version of mCherry by Shaner, N. et al (2004), derived from the Discosoma (DsRed). This protein is used to construct a FRET pair to detect DNA damage in mammalian cells. Please refer to BBa_K4814006.
Click here for the experiment data: RPA1-mCherry BBa_K4814007
References
Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N., Palmer, A. E., & Tsien, R. Y. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature biotechnology, 22(12), 1567–1572. https://doi.org/10.1038/nbt1037
Campbell, R. E., Tour, O., Palmer, A. E., Steinbach, P. A., Baird, G. S., Zacharias, D. A., & Tsien, R. Y. (2002). A monomeric red fluorescent protein. Proceedings of the National Academy of Sciences of the United States of America, 99(12), 7877–7882. https://doi.org/10.1073/pnas.082243699
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]