Difference between revisions of "Part:BBa K4814010"

Line 6: Line 6:
  
 
The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date.  
 
The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date.  
 +
 +
Click here for the experiment data: <html><a href="https://parts.igem.org/Part:BBa_K4814009">RPA1-EYFP BBa_K4814006</a></html>
  
 
References:
 
References:

Revision as of 09:51, 12 October 2023

Mammalian Codon Optimized EYFP


We have used the codon optimized version of EYFP by Watcher, R. M. et al (1998). This protein is used to construct a FRET pair to detect DNA damage in mammalian cells. Please refer to BBa_K4814009.

The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date.

Click here for the experiment data: RPA1-EYFP BBa_K4814006

References:

Wachter, R. M., Elsliger, M. A., Kallio, K., Hanson, G. T., & Remington, S. J. (1998). Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein. Structure (London, England : 1993), 6(10), 1267–1277. https://doi.org/10.1016/s0969-2126(98)00127-0

Sequence derived from: https://www.snapgene.com/plasmids/fluorescent_protein_genes_and_plasmids/EYFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]