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===Functional Parameters===
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Revision as of 09:46, 12 October 2023

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 92
    Illegal SapI.rc site found at 1044


PsrfA-sulA

This part is composed of PsrfA(BBa_k4614202), an autoregulatory promoter derived from Bacillus subtilis 168 and sulA(BBa_K4614201),which encodes SulA，an endogenous cell division inhibitor from Escherichia coli K12. Additionally, PsrfA sequence contains RBS itself, so there is no need to insert any RBS sequence during construct. CAU-China 2023 used this part to construct a cell-density-dependent expression system in E.coli (BL21) in the filamentation module of our project, thus enabling engineered bacteria to express SulA without any inducer and start filamentation process automatically. We inserted this part into pJUMP46-2A vector and obtained E.coli BL21 harboring recombinant plasmids. The recombinant strain was cultivated for over 24 hours and examined by optical microscope after stained with crystal violet.

Fig.1 Division arrest by quorum - sensing induction through PsrfA of SulA. The strain used was E.coli BL21 that carries sulA gene under PsrfA promoter.

As shown in Fig.1, it is suggested that PsrfA-sulA is sufficient to cause filamentous growth of engineered bacteria. Yet，the expression characteristic of this composite part in chassis bacteria has not been identified.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 92
    Illegal SapI.rc site found at 1044