Difference between revisions of "Part:BBa J435309"
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URL: https://freegenes.github.io/genes/BBF10K_003455.html | URL: https://freegenes.github.io/genes/BBF10K_003455.html | ||
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| + | The 3' BsaI fusion site is blocked by dcm methylation. Do not propogate in DH5a or any other dcm+ strain before use in IIs assembly. | ||
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Latest revision as of 09:45, 12 October 2023
DN4_GS flexible linker
A GS C terminal flexible linker. Defined as a DT4 part.
FLANKED 5' fusion site: AGGT, 3' fusion site: TTCG
Source organism: N/A, Target organism: Escherichia coli
ORG: E. coli Protein Expression Toolkit, Open Bioeconomy & The FreeGenes Project
URL: https://freegenes.github.io/genes/BBF10K_003455.html
The 3' BsaI fusion site is blocked by dcm methylation. Do not propogate in DH5a or any other dcm+ strain before use in IIs assembly.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]