Difference between revisions of "Part:BBa K4591009:Design"

 
(→‎References)
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===References===
 
===References===
 +
[1] Li, J., Nina, M. R. H., Zhang, X., & Bai, Y. (2022). Engineering Transcription Factor XYLS for sensing phthalic acid and terephthalic acid: an application for enzyme evolution. ACS Synthetic Biology, 11(3), 1106–1113. https://doi.org/10.1021/acssynbio.1c00275
 +
[2] Westers, L., Dijkstra, D.S., Westers, H., van Diji, J.M., Quax, W.J. (2006). "Secretion of functional human interleukin-3 from Bacillus subtilis." Journal of biotechnology 123.2 (2006): 211-224.
 +
[3] Liu S. L., Du K. (2012). Enhanced expression of an endoglucanase in Bacillus subtilis by using the sucrose-inducible sacB promoter and improved properties of the recombinant enzyme. Protein Expr Purif. 2012 Jun;83(2):164-8. doi: 10.1016/j.pep.2012.03.015. Epub 2012 Apr 4
 +
[4] Guan C., Cui W., Cheng J., Zhou L., Guo J., Hu X., Xiao G., Zhou Z. (2015). Construction and development of an auto-regulatory gene expression system in Bacillus subtilis. Microb Cell Fact. 2015; 14: 150. doi: 10.1186/s12934-015-0341-2.
 +
[5] Chen, J., Gai, Y., Fu, G., Zhou, W., Zhang, D., & Wen, J. (2015). Enhanced extracellular production of α-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein. Biotechnology letters, 37(4), 899-906.
 +
[6] Ansari, A., Ahmed, A. K., Matsangos, A. E., Lay, F., Born, L. J., Marti, G., Harmon, J. W., & Sun, Z. (2016). Cellular GFP toxicity and Immunogenicity: Potential confounders in in vivo cell tracking experiments. Stem Cell Reviews and Reports, 12(5), 553–559. https://doi.org/10.1007/s12015-016-9670-8

Revision as of 09:24, 12 October 2023


T500-RFP-lox66-Hpall-lox71-XylSmut-tetO-sfGFP-T500


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2580
    Illegal PstI site found at 2574
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2580
    Illegal PstI site found at 2574
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2580
    Illegal XhoI site found at 2261
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2580
    Illegal PstI site found at 2574
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2580
    Illegal PstI site found at 2574
    Illegal NgoMIV site found at 2255
    Illegal AgeI site found at 73
    Illegal AgeI site found at 185
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T500_RFP_rbs_lox66_Hpall_lox71_rbs_XylSmut_3tetO_rbs_sfGFP_T500


Source

T500_RFP_rbs_lox66_Hpall_lox71_rbs_XylSmut_3tetO_rbs_sfGFP_T500

References

[1] Li, J., Nina, M. R. H., Zhang, X., & Bai, Y. (2022). Engineering Transcription Factor XYLS for sensing phthalic acid and terephthalic acid: an application for enzyme evolution. ACS Synthetic Biology, 11(3), 1106–1113. https://doi.org/10.1021/acssynbio.1c00275 [2] Westers, L., Dijkstra, D.S., Westers, H., van Diji, J.M., Quax, W.J. (2006). "Secretion of functional human interleukin-3 from Bacillus subtilis." Journal of biotechnology 123.2 (2006): 211-224. [3] Liu S. L., Du K. (2012). Enhanced expression of an endoglucanase in Bacillus subtilis by using the sucrose-inducible sacB promoter and improved properties of the recombinant enzyme. Protein Expr Purif. 2012 Jun;83(2):164-8. doi: 10.1016/j.pep.2012.03.015. Epub 2012 Apr 4 [4] Guan C., Cui W., Cheng J., Zhou L., Guo J., Hu X., Xiao G., Zhou Z. (2015). Construction and development of an auto-regulatory gene expression system in Bacillus subtilis. Microb Cell Fact. 2015; 14: 150. doi: 10.1186/s12934-015-0341-2. [5] Chen, J., Gai, Y., Fu, G., Zhou, W., Zhang, D., & Wen, J. (2015). Enhanced extracellular production of α-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein. Biotechnology letters, 37(4), 899-906. [6] Ansari, A., Ahmed, A. K., Matsangos, A. E., Lay, F., Born, L. J., Marti, G., Harmon, J. W., & Sun, Z. (2016). Cellular GFP toxicity and Immunogenicity: Potential confounders in in vivo cell tracking experiments. Stem Cell Reviews and Reports, 12(5), 553–559. https://doi.org/10.1007/s12015-016-9670-8