Difference between revisions of "Part:BBa K4825048:Design"

 
Line 16: Line 16:
  
 
===References===
 
===References===
 +
1. ACS Synthetic Biology 2020 9 (3), 546-555
 +
2. "ACS Synth. Biol. 2020, 9, 3, 546–555
 +
Publication Date:February 12, 2020
 +
 +
https://doi.org/10.1021/acssynbio.9b00420"

Revision as of 09:10, 12 October 2023


COX6-LEU2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 151
    Illegal EcoRI site found at 1090
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 151
    Illegal EcoRI site found at 1090
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 151
    Illegal EcoRI site found at 1090
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 151
    Illegal EcoRI site found at 1090
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 151
    Illegal EcoRI site found at 1090
    Illegal AgeI site found at 703
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 437
    Illegal SapI.rc site found at 421


Design Notes

The composite part appends the first 41 amino acids of COX6 to the N-terminus of LEU2 in order to relocate and overexpress LEU2 in mitochondria.


Source

Saccharomyces cerevisiae

References

1. ACS Synthetic Biology 2020 9 (3), 546-555 2. "ACS Synth. Biol. 2020, 9, 3, 546–555 Publication Date:February 12, 2020

https://doi.org/10.1021/acssynbio.9b00420"