Difference between revisions of "Part:BBa K4895203"

 
 
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Often, teams may seek to test multiple enzymes independently to compare their activity. To simplify the process of creating several expression vectors we used a optogentically controlled plasmid backbone wherein the red fluorescent protein mScarlet would act as our “spacer” sequence. mScarrelt dually acts as a visible selection marker to confirm that cloning was successful or not. The mScarlet ”spacer” sequence also contains a promoter, terminator, and ribosome binding site to ensure bacterial expression.There are Bbs1 cut sites flanking the Scarlet spacer region. In combination with the accompanying golden gate template (BBa0009123) teams can easily use this construct and template to assemble multiple things in only one pcr. Successful transformants are expected to appear white.
  
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4895203 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4895203 parameters</partinfo>
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Latest revision as of 09:05, 12 October 2023

mScarlet Optogentically controlled plasmid backbone (No His Tags)

Often, teams may seek to test multiple enzymes independently to compare their activity. To simplify the process of creating several expression vectors we used a optogentically controlled plasmid backbone wherein the red fluorescent protein mScarlet would act as our “spacer” sequence. mScarrelt dually acts as a visible selection marker to confirm that cloning was successful or not. The mScarlet ”spacer” sequence also contains a promoter, terminator, and ribosome binding site to ensure bacterial expression.There are Bbs1 cut sites flanking the Scarlet spacer region. In combination with the accompanying golden gate template (BBa0009123) teams can easily use this construct and template to assemble multiple things in only one pcr. Successful transformants are expected to appear white.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 683
    Illegal NgoMIV site found at 815
    Illegal NgoMIV site found at 909
    Illegal NgoMIV site found at 1202
    Illegal NgoMIV site found at 1696
    Illegal NgoMIV site found at 1714
    Illegal NgoMIV site found at 1804
    Illegal AgeI site found at 1034
    Illegal AgeI site found at 2162
    Illegal AgeI site found at 3946
  • 1000
    COMPATIBLE WITH RFC[1000]