Difference between revisions of "Part:BBa K4630006:Design"

(→‎References)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
Because the Cas9 sequence in origin part is not consistent with our plasmid, so we found this part.
+
Because the Cas9 sequence in original part is not consistent with our plasmid, so we found this part.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Latest revision as of 09:04, 12 October 2023


Cas9 in pCas plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1344
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1344
    Illegal NheI site found at 1103
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1344
    Illegal BamHI site found at 3382
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1344
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1344
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because the Cas9 sequence in original part is not consistent with our plasmid, so we found this part.

Source

Zhao, D., Yuan, S., Xiong, B. et al. Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9. Microb Cell Fact 15, 205 (2016).

References

Zhao, D., Yuan, S., Xiong, B. et al. Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9. Microb Cell Fact 15, 205 (2016)