Difference between revisions of "Part:BBa K4825046:Design"
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===References=== | ===References=== | ||
+ | "1. Hammer SK, Zhang Y, Avalos JL. Mitochondrial Compartmentalization Confers Specificity to the 2-Ketoacid Recursive Pathway: Increasing Isopentanol Production in Saccharomyces cerevisiae. ACS Synth Biol. 2020 Mar 20;9(3):546-555. doi: 10.1021/acssynbio.9b00420. Epub 2020 Feb 21. PMID: 32049515. | ||
+ | 2. Yuan J, Chen X, Mishra P, Ching CB. Metabolically engineered Saccharomyces cerevisiae for enhanced isoamyl alcohol production. Appl Microbiol Biotechnol. 2017 Jan;101(1):465-474. doi: 10.1007/s00253-016-7970-1. Epub 2016 Nov 15. PMID: 27847988." | ||
+ | |||
+ | Verstrepen KJ, Van Laere SD, Vanderhaegen BM, Derdelinckx G, Dufour JP, Pretorius IS, Winderickx J, Thevelein JM, Delvaux FR. Expression levels of the yeast alcohol acetyltransferase genes ATF1, Lg-ATF1, and ATF2 control the formation of a broad range of volatile esters. Appl Environ Microbiol. 2003 Sep;69(9):5228-37. doi: 10.1128/AEM.69.9.5228-5237.2003. PMID: 12957907; PMCID: PMC194970. |
Revision as of 09:00, 12 October 2023
ARO10-ADH7-ATF1
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1791
Illegal EcoRI site found at 3418
Illegal SpeI site found at 742
Illegal PstI site found at 620 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1791
Illegal EcoRI site found at 3418
Illegal NheI site found at 2385
Illegal SpeI site found at 742
Illegal PstI site found at 620 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1791
Illegal EcoRI site found at 3418
Illegal BglII site found at 3105
Illegal BamHI site found at 2146
Illegal BamHI site found at 4432 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1791
Illegal EcoRI site found at 3418
Illegal SpeI site found at 742
Illegal PstI site found at 620 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1791
Illegal EcoRI site found at 3418
Illegal SpeI site found at 742
Illegal PstI site found at 620 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3532
Illegal SapI.rc site found at 4540
Design Notes
By overexpression of ARO10 and ADH7, the supply of precursors isopenaldehyde and isopentanol is increased, and the overexpression of ATF1 directly enhances the corresponding conversion to isoamyl acetate. Therefore, The composite part increases the production of isoamyl acetate in S. cerevisiae.
Source
Saccharomyces cerevisiae
References
"1. Hammer SK, Zhang Y, Avalos JL. Mitochondrial Compartmentalization Confers Specificity to the 2-Ketoacid Recursive Pathway: Increasing Isopentanol Production in Saccharomyces cerevisiae. ACS Synth Biol. 2020 Mar 20;9(3):546-555. doi: 10.1021/acssynbio.9b00420. Epub 2020 Feb 21. PMID: 32049515. 2. Yuan J, Chen X, Mishra P, Ching CB. Metabolically engineered Saccharomyces cerevisiae for enhanced isoamyl alcohol production. Appl Microbiol Biotechnol. 2017 Jan;101(1):465-474. doi: 10.1007/s00253-016-7970-1. Epub 2016 Nov 15. PMID: 27847988."
Verstrepen KJ, Van Laere SD, Vanderhaegen BM, Derdelinckx G, Dufour JP, Pretorius IS, Winderickx J, Thevelein JM, Delvaux FR. Expression levels of the yeast alcohol acetyltransferase genes ATF1, Lg-ATF1, and ATF2 control the formation of a broad range of volatile esters. Appl Environ Microbiol. 2003 Sep;69(9):5228-37. doi: 10.1128/AEM.69.9.5228-5237.2003. PMID: 12957907; PMCID: PMC194970.