Difference between revisions of "Part:BBa K4759206"

Latest revision as of 08:51, 12 October 2023

T7-RBS4-TF-linker-OleP

We add a TF(solubilization-promoting tag) at the N-terminal of the P450 enzyme

Usage and Biology

We add a solubilization-promoting tag at the N-terminal of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.

Fig1: The application of protein tags

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 591
Illegal AgeI site found at 1428
Illegal AgeI site found at 1526
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 159
Illegal SapI.rc site found at 807
Illegal SapI.rc site found at 920
Illegal SapI.rc site found at 1176

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4759206 short</partinfo>
-We fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.
+We add a TF(solubilization-promoting tag) at the N-terminal of the P450 enzyme
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+We add a solubilization-promoting tag at the N-terminal of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.
+https://static.igem.wiki/teams/4759/wiki/protein-tags.png
+Fig1: The application of protein tags
 <!-- -->