Difference between revisions of "Part:BBa K4645008"
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===Result=== | ===Result=== | ||
| + | <p>We used homologous recombination in which the OmpA signal peptide was added to the N-terminus of ClyR and transformed into <i>E.coli</i> DH5α competent cells and incubated overnight at 37℃. Below is a plot of colony PCR results.</p> | ||
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<center><b>Figure 1.</b> Colony PCR assay of the OmpA-ClyR fragment. </center> | <center><b>Figure 1.</b> Colony PCR assay of the OmpA-ClyR fragment. </center> | ||
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===Sequence and Features=== | ===Sequence and Features=== | ||
<partinfo>BBa_K4645008 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4645008 SequenceAndFeatures</partinfo> | ||
Revision as of 08:50, 12 October 2023
A chimeric lysozyme with secretory ability.
ClyR is a chimeric lysin, comprising a cysteine, histidine-dependent amidohydrolase/peptidase catalytic domain from PlyC lysin, and the cell-wall binding domain from PlySs2 lysin. The signal peptide OmpA enables ClyR to be secreted into the periplasmic space of bacteria.
Result
We used homologous recombination in which the OmpA signal peptide was added to the N-terminus of ClyR and transformed into E.coli DH5α competent cells and incubated overnight at 37℃. Below is a plot of colony PCR results.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 816
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 184
Illegal AgeI site found at 268
Illegal AgeI site found at 583
Illegal AgeI site found at 766 - 1000COMPATIBLE WITH RFC[1000]