Difference between revisions of "Part:BBa K4759208"
 
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<partinfo>BBa_K4759208 short</partinfo>
 
<partinfo>BBa_K4759208 short</partinfo>
  
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NusA protein (N-utilization substance A) is a class of transcription elongation antiterminator in E. coli with a molecular weight of about 55 kDa.
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NusA protein (N-utilization substance A) is a class of transcription elongation antiterminator in E. coli with a molecular weight of about 55 kDa.<br>
 
We fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.
 
We fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.
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https://static.igem.wiki/teams/4759/wiki/protein-tags.png
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Fig1: The application of protein tags
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:41, 12 October 2023


T7-RBS4-Nus-linker-Olep

NusA protein (N-utilization substance A) is a class of transcription elongation antiterminator in E. coli with a molecular weight of about 55 kDa.
We fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.

protein-tags.png

Fig1: The application of protein tags


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1463
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1623
    Illegal AgeI site found at 1721
  • 1000
    COMPATIBLE WITH RFC[1000]