Difference between revisions of "Part:BBa K4759208"
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<partinfo>BBa_K4759208 short</partinfo>
 
<partinfo>BBa_K4759208 short</partinfo>
  
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we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.
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NusA protein (N-utilization substance A) is a class of transcription elongation antiterminator in E. coli with a molecular weight of about 55 kDa.
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We fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 08:41, 12 October 2023


T7-RBS4-Nus-linker-Olep

NusA protein (N-utilization substance A) is a class of transcription elongation antiterminator in E. coli with a molecular weight of about 55 kDa. We fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1463
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1623
    Illegal AgeI site found at 1721
  • 1000
    COMPATIBLE WITH RFC[1000]