Difference between revisions of "Part:BBa K4670134"
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We successfully produced recombinant plasmid, with 3563 DNA base pairs. By the process of transformation and Golden gate assembly, we have successfully bioengineered a kind of bacteria, which can produce the protein that we want. The following picture indicates that the incubated bacteria, which was on an agar plate and it was stored for further usage. We intentionally added antibiotics in the agar plate to test whether the bacteria can be incubated successfully. If it can’t be killed by those specific antibiotics, it means that the bacteria is the specific bacteria with our recombinant plasmid. | We successfully produced recombinant plasmid, with 3563 DNA base pairs. By the process of transformation and Golden gate assembly, we have successfully bioengineered a kind of bacteria, which can produce the protein that we want. The following picture indicates that the incubated bacteria, which was on an agar plate and it was stored for further usage. We intentionally added antibiotics in the agar plate to test whether the bacteria can be incubated successfully. If it can’t be killed by those specific antibiotics, it means that the bacteria is the specific bacteria with our recombinant plasmid. | ||
To prove that the modified bacteria has our designed gene, we used gel electrophoresis to check whether the bacteria contains our gene or not. In the picture below, we can see the band of our bacteria at the position around 3563 base pairs of the ladder, which is exactly the length of our gene. This indicates that the bacteria contains our designed gene. | To prove that the modified bacteria has our designed gene, we used gel electrophoresis to check whether the bacteria contains our gene or not. In the picture below, we can see the band of our bacteria at the position around 3563 base pairs of the ladder, which is exactly the length of our gene. This indicates that the bacteria contains our designed gene. | ||
| + | <img class="img-fluid" src="https://static.igem.wiki/teams/4670/wiki/result02.png" style="width:100%"> | ||
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Revision as of 08:35, 12 October 2023
Encodes a member of the GATA factor family of zinc finger transcription factors.
GATA4 is a member of the GATA family of zinc finger transcription factor, which regulates gene transcription by binding to GATA elements. GATA4 was originally discovered as a regulator of cardiac development and subsequently identified as a major regulator of adult cardiac hypertrophy. GATA4 is expressed in both embryo and adult cardiomyocytes where it functions as a transcriptional regulator for many cardiac genes, and also regulates hypertrophic growth of the heart. GATA4 promotes cardiac morphogenesis, cardiomyocytes survival, and maintains cardiac function in the adult heart. We aim to use GATA 4 transcription factor to synthesize a fusion protein to enter the cell by endocytosis, in order to suppress the activation of Hepatic stellate cell. Thus, the symptoms consequential with primary chronic liver disease is suppressed by deactivating active HSCs.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 197
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 197
Illegal NheI site found at 283 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 197
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 197
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 197
- 1000COMPATIBLE WITH RFC[1000]