Difference between revisions of "Part:BBa K4837001"
Line 23: Line 23:
  
 
<html>
 
<html>
−
<a href='https://static.igem.wiki/teams/4837/wiki/description-photo-4.png' target="_blank"><img src="https://static.igem.wiki/teams/4837/wiki/description-photo-4.pngg" width="30%"/>
+
<a href='https://static.igem.wiki/teams/4837/wiki/description-photo-4.png' target="_blank"><img src="https://static.igem.wiki/teams/4837/wiki/description-photo-4.png" width="30%"/>
 
</a>
 
</a>
 
</html>
 
</html>

Revision as of 08:34, 12 October 2023


Manganese peroxidase

This plasmid optimized the sequence from white-rot fungus (Phanerochaete chrysosporium) for E. coli expression.


The product illustrated below, namely manganese peroxidase (MnP), works together with a DNA duplex and another product from BBa_K4837000 to form a "cascade enzyme system" for PFAs degradation.

MnP is conjugated with an azide-modified DNA oligo, which is illustrated below:

Finally, the "cascade enzyme system" formed when two DNA oligos in the conjugated products bound with each other by complementary base pairing principle, which is illustrated below:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 126
    Illegal NotI site found at 781
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 66
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1149
    Illegal NgoMIV site found at 1153
    Illegal AgeI site found at 651
    Illegal AgeI site found at 1132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 865
    Illegal SapI.rc site found at 523