Difference between revisions of "Part:BBa K4645008"
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<p>We used homologous recombination in which the OmpA signal peptide was added to the N-terminus of ClyR and transformed into <i>E.coli</i> DH5α competent cells and incubated overnight at 37℃. Below is a plot of colony PCR results.</p> | <p>We used homologous recombination in which the OmpA signal peptide was added to the N-terminus of ClyR and transformed into <i>E.coli</i> DH5α competent cells and incubated overnight at 37℃. Below is a plot of colony PCR results.</p> | ||
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| + | <center><img src="https://static.igem.wiki/teams/4645/wiki/wet-lab/clyr/ompa-clyr-p.png" style="width:40%; "></center> | ||
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| − | + | <center><b>Figure 1.</b> Colony PCR assay of the OmpA-ClyR fragment. </center> | |
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| − | <center><b>Figure 1.</b> Colony PCR assay of the OmpA-ClyR fragment.</center> | + | |
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Revision as of 08:33, 12 October 2023
A chimeric lysozyme with secretory ability.
ClyR is a chimeric lysin, comprising a cysteine, histidine-dependent amidohydrolase/peptidase catalytic domain from PlyC lysin, and the cell-wall binding domain from PlySs2 lysin. The signal peptide OmpA enables ClyR to be secreted into the periplasmic space of bacteria.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 816
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 184
Illegal AgeI site found at 268
Illegal AgeI site found at 583
Illegal AgeI site found at 766 - 1000COMPATIBLE WITH RFC[1000]