Difference between revisions of "Part:BBa K4837001"
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<partinfo>BBa_K4837001 short</partinfo> | <partinfo>BBa_K4837001 short</partinfo> | ||
| − | + | This plasmid optimized the sequence from white-rot fungus (Phanerochaete chrysosporium) for E. coli expression. | |
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| + | The product illustrated below, namely manganese peroxidase (MnP), works together with a DNA duplex and another product from BBa_K4837000 to form a "cascade enzyme system" for PFAs degradation. | ||
<html> | <html> | ||
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</a> | </a> | ||
| + | MnP is conjugated with an azide-modified DNA oligo, which is illustrated below: | ||
<html> | <html> | ||
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</a> | </a> | ||
</html> | </html> | ||
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| + | Finally, the "cascade enzyme system" formed when two DNA oligos in the conjugated products bound with each other by complementary base pairing principle, which is illustrated below: | ||
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| + | <html> | ||
| + | <a href='https://static.igem.wiki/teams/4837/wiki/description-photo-4.png' target="_blank"><img src="https://static.igem.wiki/teams/4837/wiki/description-photo-4.pngg" width="30%"/> | ||
| + | </a> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 08:32, 12 October 2023
Manganese peroxidase
This plasmid optimized the sequence from white-rot fungus (Phanerochaete chrysosporium) for E. coli expression.
The product illustrated below, namely manganese peroxidase (MnP), works together with a DNA duplex and another product from BBa_K4837000 to form a "cascade enzyme system" for PFAs degradation.
MnP is conjugated with an azide-modified DNA oligo, which is illustrated below:
Finally, the "cascade enzyme system" formed when two DNA oligos in the conjugated products bound with each other by complementary base pairing principle, which is illustrated below:
