Difference between revisions of "Part:BBa K4759207"

Latest revision as of 08:28, 12 October 2023

T7-RBS4-Trx-linker-Olep

Thioredoxin (Trx) is a series of oxidoreductases found in organisms that reduce disulfide binding by displacing thiodisulfide.
We add a solubilization-promoting tag at the N-teminal of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.

Fig1: The application of protein tags

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 411
Illegal AgeI site found at 459
Illegal AgeI site found at 557
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4759207 short</partinfo>
-we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.
+Thioredoxin (Trx) is a series of oxidoreductases found in organisms that reduce disulfide binding by displacing thiodisulfide.<br>
+We add a solubilization-promoting tag at the N-teminal of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.
+https://static.igem.wiki/teams/4759/wiki/protein-tags.png
+Fig1: The application of protein tags
 <!-- Add more about the biology of this part here