Difference between revisions of "Part:BBa K4759017"

Revision as of 08:24, 12 October 2023

Nus

NusA protein (N-utilization substance A) is a class of transcription elongation antiterminator in E. coli with a molecular weight of about 55 kDa. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1371
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4759017 short</partinfo>
-Recombinant protein expression plays an important role in promoting the development of modern biological technology. By using the host to achieve exogenous protein expression, it provides a powerful tool for studying the structure and function of some important proteins, especially the human source proteins. In order to enable subsequent structure-function studies, the obtained recombinant proteins must be soluble, stable, properly folded as well as biologically active. However, in practical studies, these often become obstacles to obtaining an ideal recombinant protein. The solubility of the recombinant protein expression became the primary addressed problem.Because NusA has the biological activity to promote DNA transcription and slow translation, the expressed protein has more time to fold, allowing the recombinant protein to be stable and active. Even without removing the NusA tag, the fusion protein can still be active.
+NusA protein (N-utilization substance A) is a class of transcription elongation antiterminator in E. coli with a molecular weight of about 55 kDa.
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 ===Usage and Biology===