Difference between revisions of "Part:BBa K4759206"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4759206 short</partinfo>
 
<partinfo>BBa_K4759206 short</partinfo>
 +
 
we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.
 
we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.
  

Revision as of 07:38, 12 October 2023


T7-RBS4-TF-linker-OleP

we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 591
    Illegal AgeI site found at 1428
    Illegal AgeI site found at 1526
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 159
    Illegal SapI.rc site found at 807
    Illegal SapI.rc site found at 920
    Illegal SapI.rc site found at 1176