Difference between revisions of "Part:BBa K4759207"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4759207 short</partinfo>
 
<partinfo>BBa_K4759207 short</partinfo>
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we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.
 
we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.
  

Revision as of 07:38, 12 October 2023


T7-RBS4-Trx-linker-Olep . we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 411
    Illegal AgeI site found at 459
    Illegal AgeI site found at 557
  • 1000
    COMPATIBLE WITH RFC[1000]