Difference between revisions of "Part:BBa K4765134"
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====Agarose gel electrophoresis==== | ====Agarose gel electrophoresis==== | ||
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| − | | <html><img style="width: | + | | <html><img style="width:200px" src="https://static.igem.wiki/teams/4765/wiki/zsl/dna-gel/fen1-ssb-tdp-xrcc1.png" alt="contributed by Fudan iGEM 2023"></html> |
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| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures. | | '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures. | ||
Revision as of 07:04, 12 October 2023
ribozyme connected: Dsup + FEN1 + H. ex mtSSB + SAHS 33020 + XRCC1
Usage and Biology
This part is the combination of all the anti-desiccation and anti-UV proteins, we've transformed it to E .coli BL21 DE3 and test its performance.
Characterization
Agarose gel electrophoresis
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| Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.
From left lane(1) to right lane(2) indicate the successful construction of H. ex mtSSB and H. ex mtSSB + SAHS 33020. |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4573
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1886
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2464
Illegal NgoMIV site found at 2782
Illegal AgeI site found at 230
Illegal AgeI site found at 301
Illegal AgeI site found at 1570 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3415
Illegal BsaI.rc site found at 227
Illegal BsaI.rc site found at 3050
Illegal BsaI.rc site found at 3131
Illegal BsaI.rc site found at 5623
Illegal SapI site found at 2944
Illegal SapI.rc site found at 956