Difference between revisions of "Part:BBa K4765121"

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====Agarose gel electrophoresis====
 
====Agarose gel electrophoresis====
 
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| <html><img style="width:640px" src="https://static.igem.wiki/teams/4765/wiki/zsl/dna-gel/gal-pgm-mscarlet.png" alt="contributed by Fudan iGEM 2023"></html>
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| <html><img style="width:200px" src="https://static.igem.wiki/teams/4765/wiki/zsl/dna-gel/gal-pgm-mscarlet.png" alt="contributed by Fudan iGEM 2023"></html>
 
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| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.  
 
| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.  

Revision as of 06:38, 12 October 2023


ribozyme connected: galU + pgmA + mScarlet contributed by Fudan iGEM 2023


Introduction

This composite part is designed for the production of exopolysaccharide (EPS), and detection of EPS’s adhesion ability. It includes E. coli galU on the upstream site and E. coli pgmA on the downstream site, two essential enzymes in EPS biosynthesis pathway [1]. These two enzymes were tested by iGEM20_XJTU-China in K3331001(galU)and K3331002(pgmA). The following mScarlet is utilized to detect E. coli 's adhesion ability.

Usage and Biology

We use this part to improve EPS production of E. coli and assessment of EPS’s adhesion ability.

Characterization

Agarose gel electrophoresis

contributed by Fudan iGEM 2023
Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.

From left lane(1) to right lane(3) indicate the successful construction of galU, galU + pgmA, and galU + pgmA + mScarlet.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3285
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. 需要参考文献