Difference between revisions of "Part:BBa K4765117"
| Line 13: | Line 13: | ||
====Agarose gel electrophoresis==== | ====Agarose gel electrophoresis==== | ||
{| | {| | ||
| − | | <html><img style="width: | + | | <html><img style="width:200px" src="https://static.igem.wiki/teams/4765/wiki/zsl/dna-gel/ssb-tdp.png" alt="contributed by Fudan iGEM 2023"></html> |
|- | |- | ||
| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures. | | '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures. | ||
Revision as of 06:37, 12 October 2023
ribozyme connected: H. ex mtSSB + SAHS 33020
Introduction
This composite part is composed of BBa_K4765112 and BBa_K4765113.The last stem-loop is replaced by T7 terminator.
Usage and Biology
We introduced this part into E. coli, enabling the heterologous expression of both mtSSB and TDP proteins, to assess the combined desiccation resistance capability of these two proteins.
Characterization
Agarose gel electrophoresis
|
| Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.
From left lane(1) to right lane(2) indicate the successful construction of H. ex mtSSB and H. ex mtSSB + SAHS 33020. |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 247
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 880
Illegal BsaI.rc site found at 515
Illegal BsaI.rc site found at 596
Illegal SapI site found at 409