Difference between revisions of "Part:BBa K4765117"
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We introduced this part into ''E. coli'', enabling the heterologous expression of both mtSSB and TDP proteins, to assess the combined desiccation resistance capability of these two proteins.
 
We introduced this part into ''E. coli'', enabling the heterologous expression of both mtSSB and TDP proteins, to assess the combined desiccation resistance capability of these two proteins.
 
===Characterization===
 
===Characterization===
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====Agarose gel electrophoresis====
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{|
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| <html><img style="width:640px" src="https://static.igem.wiki/teams/4765/wiki/zsl/dna-gel/ssb-tdp.png" alt="contributed by Fudan iGEM 2023"></html>
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|-
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| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.
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From left lane(1) to right lane(2) indicate the successful construction of H. ex mtSSB and H. ex mtSSB + SAHS 33020. '''
  
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|}
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 06:35, 12 October 2023


ribozyme connected: H. ex mtSSB + SAHS 33020

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of BBa_K4765112 and BBa_K4765113.The last stem-loop is replaced by T7 terminator.

Usage and Biology

We introduced this part into E. coli, enabling the heterologous expression of both mtSSB and TDP proteins, to assess the combined desiccation resistance capability of these two proteins.

Characterization

Agarose gel electrophoresis

contributed by Fudan iGEM 2023
Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.

From left lane(1) to right lane(2) indicate the successful construction of H. ex mtSSB and H. ex mtSSB + SAHS 33020.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 880
    Illegal BsaI.rc site found at 515
    Illegal BsaI.rc site found at 596
    Illegal SapI site found at 409