Difference between revisions of "Part:BBa K4664000"
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4664000 short</partinfo>
 
<partinfo>BBa_K4664000 short</partinfo>
Line 17: Line 16:
 
<partinfo>BBa_K4664000 parameters</partinfo>
 
<partinfo>BBa_K4664000 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
===Background===
 +
miR-223 promotes TGF (transforming growth factor)- β1 expression, the activated signalling of which is considered to be of great importance in the pathogenesis of emphysema, one of the two pathophysiological branches of COPD.
 +
<br>
 +
miR-223 is significantly upregulated in COPD patients (Roffel et al., 2021).
 +
<br>
 +
RT-qPCR (Reverse Transcription Quantitative Real-time Polymerase Chain Reaction), is a combination of RT-PCR and qPCR methods, and is commonly employed for the detection and quantification of RNA. The procedure involves the enzyme reverse transcriptase converting total RNA or messenger RNA (mRNA) to complementary DNA (cDNA). This cDNA is then amplified and used in quantitative PCR (qPCR) or real-time PCR to detect specific targets. This PCR method utilises a number of fluorescent chemicals to quantify the amount of DNA at each cycle in real-time.
 +
<br>
 +
===Design===
 +
miRNA sequence:
 +
<br>CGUGUAUUUGACAAGCUGAGUU
 +
===Results===
 +
The following charts show the RT-qPCR results for miR-223.
 +
<br>
 +
https://static.igem.wiki/teams/4664/wiki/part/p464000-1.png
 +
<br>
 +
<i>Figure 1. Results for miR-223 RT-qPCR.</i>
 +
<br>
 +
===References===
 +
Roffel, M. P., Maes, T., Brandsma, C., Van Den Berge, M., Vanaudenaerde, B. M., Joos, G., Brusselle, G., Heijink, I. H., & Bracke, K. (2021). MiR-223 is increased in lungs of patients with COPD and modulates cigarette smoke-induced pulmonary inflammation. American Journal of Physiology-lung Cellular and Molecular Physiology, 321(6), L1091–L1104. https://doi.org/10.1152/ajplung.00252.2021

Latest revision as of 05:52, 12 October 2023

miRNA for COPD

MiRNA-223 is the biomarker that promotes transforming growth factor (TGF)-β1 expression, and TGF-β1 augments miR-223 expression. It is related to the presence of COPD.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Background

miR-223 promotes TGF (transforming growth factor)- β1 expression, the activated signalling of which is considered to be of great importance in the pathogenesis of emphysema, one of the two pathophysiological branches of COPD.
miR-223 is significantly upregulated in COPD patients (Roffel et al., 2021).
RT-qPCR (Reverse Transcription Quantitative Real-time Polymerase Chain Reaction), is a combination of RT-PCR and qPCR methods, and is commonly employed for the detection and quantification of RNA. The procedure involves the enzyme reverse transcriptase converting total RNA or messenger RNA (mRNA) to complementary DNA (cDNA). This cDNA is then amplified and used in quantitative PCR (qPCR) or real-time PCR to detect specific targets. This PCR method utilises a number of fluorescent chemicals to quantify the amount of DNA at each cycle in real-time.

Design

miRNA sequence:
CGUGUAUUUGACAAGCUGAGUU

Results

The following charts show the RT-qPCR results for miR-223.
p464000-1.png
Figure 1. Results for miR-223 RT-qPCR.

References

Roffel, M. P., Maes, T., Brandsma, C., Van Den Berge, M., Vanaudenaerde, B. M., Joos, G., Brusselle, G., Heijink, I. H., & Bracke, K. (2021). MiR-223 is increased in lungs of patients with COPD and modulates cigarette smoke-induced pulmonary inflammation. American Journal of Physiology-lung Cellular and Molecular Physiology, 321(6), L1091–L1104. https://doi.org/10.1152/ajplung.00252.2021