Difference between revisions of "Part:BBa K4893001"
 
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<partinfo>BBa_K4893001 short</partinfo>
 
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A.calcoaceticus derived PQQGDH is a dimeric enzyme which is recombinantly produced using  E. coli as a host microorganism. It is responsible for oxidizing glucose into glucono-δ-lactone which is then hydrolyzed into gluconic acid. In PQQGDH holo form, it possesses the PQQ cofactor that gives its redox abilities. This enzyme has been most widely used for glucose-sensing applications. In our project, we present a new application of this enzyme in pyocyanin sensing for P. aeruginosa detection.  
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A.calcoaceticus derived PQQGDH is a dimeric enzyme which is recombinantly produced using  E. coli. It is responsible for oxidizing glucose into glucono-δ-lactone which is then hydrolyzed into gluconic acid. In PQQGDH holo form, it possesses the PQQ cofactor that gives the protein its electron-carrying abilities. This enzyme has been most widely used for glucose-sensing applications. In our project, we present a new application of this enzyme in pyocyanin sensing for P. aeruginosa detection.  
  
 
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<partinfo>BBa_K4893001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4893001 SequenceAndFeatures</partinfo>
  
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SDS page of Crude Sample of PQQGDH
 
https://static.igem.wiki/teams/4893/wiki/bba-k4893001-sds-page.jpg
 
https://static.igem.wiki/teams/4893/wiki/bba-k4893001-sds-page.jpg
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Modeling of PQQGDH in PyMOL
 
https://static.igem.wiki/teams/4893/wiki/bba-k4893001-model.jpg
 
https://static.igem.wiki/teams/4893/wiki/bba-k4893001-model.jpg
  

Latest revision as of 05:50, 12 October 2023


Pyrroloquinoline Quinone Glucose Dehydrogenase

A.calcoaceticus derived PQQGDH is a dimeric enzyme which is recombinantly produced using E. coli. It is responsible for oxidizing glucose into glucono-δ-lactone which is then hydrolyzed into gluconic acid. In PQQGDH holo form, it possesses the PQQ cofactor that gives the protein its electron-carrying abilities. This enzyme has been most widely used for glucose-sensing applications. In our project, we present a new application of this enzyme in pyocyanin sensing for P. aeruginosa detection.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 450
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

SDS page of Crude Sample of PQQGDH bba-k4893001-sds-page.jpg

Modeling of PQQGDH in PyMOL bba-k4893001-model.jpg