Difference between revisions of "Part:BBa K4893001:Experience"
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how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
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We tested to determine whether pyocyanin could be an electron acceptor of the PQQGDH and glucose oxidation reaction for the purpose of a pyocyanin biosensor. The gene was successfully expressed by Escherichia coli BL21 DE3 cells and tested with PQQGDH, although results were inconclusive and require further activity testing.  
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We tested to determine whether pyocyanin could be an electron acceptor of the PQQGDH and glucose oxidation reaction for the purpose of a pyocyanin biosensor. The protein was successfully expressed by Escherichia coli BL21 DE3 cells and tested with PQQGDH, although results were inconclusive and require further activity testing. One recommendation is to incubated PQQGDH with Ca2+ ions to improve the metal-ion coordinate binding between the GDH and PQQ cofactor.  
  
 
===Applications of BBa_K4893001===
 
===Applications of BBa_K4893001===
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Glucose sensors, enzyme fuel cells
  
 
===User Reviews===
 
===User Reviews===

Revision as of 05:44, 12 October 2023


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

We tested to determine whether pyocyanin could be an electron acceptor of the PQQGDH and glucose oxidation reaction for the purpose of a pyocyanin biosensor. The protein was successfully expressed by Escherichia coli BL21 DE3 cells and tested with PQQGDH, although results were inconclusive and require further activity testing. One recommendation is to incubated PQQGDH with Ca2+ ions to improve the metal-ion coordinate binding between the GDH and PQQ cofactor.

Applications of BBa_K4893001

Glucose sensors, enzyme fuel cells

User Reviews

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