Difference between revisions of "Part:BBa K4893001:Design"
 
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===Design Notes===
 
===Design Notes===
  
−
 
+
The replacement of Ser231 to Lys resulted in an increase in thermal stability.[1]
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+
  
 
===Source===
 
===Source===
  
−
 
+
Acinetobacter calcoaceticus
  
 
===References===
 
===References===
  
−
Sode K, Ootera T, Shirahane M, Witarto AB, Igarashi S, Yoshida H. Increasing the thermal stability of the water-soluble pyrroloquinoline quinone glucose dehydrogenase by single amino acid replacement. Enzyme Microb Technol. 2000 Apr 1;26(7):491-496. doi: 10.1016/s0141-0229(99)00196-9. PMID: 10771051.
+
[1] Sode K, Ootera T, Shirahane M, Witarto AB, Igarashi S, Yoshida H. Increasing the thermal stability of the water-soluble pyrroloquinoline quinone glucose dehydrogenase by single amino acid replacement. Enzyme Microb Technol. 2000 Apr 1;26(7):491-496. doi: 10.1016/s0141-0229(99)00196-9. PMID: 10771051.
  
−
Igarashi S, Sode K. Stabilization of quaternary structure of water-soluble quinoprotein glucose dehydrogenase. Mol Biotechnol. 2003 Jun;24(2):97-104. doi: 10.1385/MB:24:2:97. PMID: 12746550.
+
[2] Igarashi S, Sode K. Stabilization of quaternary structure of water-soluble quinoprotein glucose dehydrogenase. Mol Biotechnol. 2003 Jun;24(2):97-104. doi: 10.1385/MB:24:2:97. PMID: 12746550.

Latest revision as of 05:36, 12 October 2023


Pyrroloquinoline Quinone Glucose Dehydrogenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 450
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The replacement of Ser231 to Lys resulted in an increase in thermal stability.[1]

Source

Acinetobacter calcoaceticus

References

[1] Sode K, Ootera T, Shirahane M, Witarto AB, Igarashi S, Yoshida H. Increasing the thermal stability of the water-soluble pyrroloquinoline quinone glucose dehydrogenase by single amino acid replacement. Enzyme Microb Technol. 2000 Apr 1;26(7):491-496. doi: 10.1016/s0141-0229(99)00196-9. PMID: 10771051.

[2] Igarashi S, Sode K. Stabilization of quaternary structure of water-soluble quinoprotein glucose dehydrogenase. Mol Biotechnol. 2003 Jun;24(2):97-104. doi: 10.1385/MB:24:2:97. PMID: 12746550.