Difference between revisions of "Part:BBa K4893001"
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Revision as of 05:28, 12 October 2023
Pyrroloquinoline Quinone Glucose Dehydrogenase
A.calcoaceticus derived PQQGDH is a dimeric enzyme which is recombinantly produced using E. coli as a host microorganism. It is responsible for oxidizing glucose into glucono-δ-lactone which is then hydrolyzed into gluconic acid. In PQQGDH holo form, it possesses the PQQ cofactor that gives its redox abilities. This enzyme has been most widely used for glucose-sensing applications. In our project, we present a new application of this enzyme in pyocyanin sensing for P. aeruginosa detection.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 450
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]