Difference between revisions of "Part:BBa K283014"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K283014 short</partinfo>
 
<partinfo>BBa_K283014 short</partinfo>
  
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prosseA promoter with a rbs. This part is similar to K283012, but with a different plasmid backbone pSB3C5
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We intended to use this device to control the expression level of cheZ.BBa_K094141 was used to repress pLacI promoter. But we failed because of the high leaky expression.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:01, 20 October 2009

pLacIq-rbs-lacI-T-pLacI-rbs-cheZ_histag-double terminators

We intended to use this device to control the expression level of cheZ.BBa_K094141 was used to repress pLacI promoter. But we failed because of the high leaky expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2196
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1261
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]