Difference between revisions of "Part:BBa K239006"

Revision as of 15:45, 20 October 2009

Modified NarK promoter

Sequence contains 2 Fnr (one of which is modified), 1 Fis, 2 NarL binding sites and initiates transcription by RNAP sigma-70. Function: Switched on during anaerobic conditions. Area of application: Detection of when the cell is switching to anaerobic metabolism. It is designed to have better affinity to activated Fnr (than BBa_K239005) and hence also stronger activity under anaerobic conditions.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 15:44, 20 October 2009 (view source) Axelnystrom (Talk \| contribs) ← Older edit		Revision as of 15:45, 20 October 2009 (view source) Axelnystrom (Talk \| contribs) Newer edit →
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	Sequence contains 2 Fnr (one of which is modified), 1 Fis, 2 NarL binding sites and initiates transcription by RNAP sigma-70. Function: Switched on during anaerobic conditions. Area of application: Detection of when the cell is switching to anaerobic metabolism.		Sequence contains 2 Fnr (one of which is modified), 1 Fis, 2 NarL binding sites and initiates transcription by RNAP sigma-70. Function: Switched on during anaerobic conditions. Area of application: Detection of when the cell is switching to anaerobic metabolism.
−	It is designed to have better affinity to activated Fnr and hence stronger activity under anaerobic conditions.	+	It is designed to have better affinity to activated Fnr (than BBa_K239005) and hence also stronger activity under anaerobic conditions.